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electrotransfer tank  (Bio-Rad)


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    Structured Review

    Bio-Rad electrotransfer tank
    Electrotransfer Tank, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrotransfer+buffer/Buffer+Tank/pmc11971999-72-8-19
    Average 96 stars, based on 251 article reviews
    electrotransfer tank - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Electrotransfer:

    Article Title: The physiological role of fat body and muscle tissues in response to cold stress in the tropical cockroach Gromphadorhina coquereliana
    Article Snippet: For each line was loaded 50 μg of proteins suspended in Laemmli sample buffer (BioRad) boiled for 5 min. Electrophoresis was performed for 1h with voltage 200 mV in electrophoretic buffer (2.5 mM Tris-HCl, 0.02 M glycine, 0.01% SDS) with electrophoresis apparatus (BioRad). .. Electrotransfer was carried out for 70 minutes in electrotransfer buffer (20% methanol, 0.02% SDS, 20 mM Tris-HCl, 0.15 M glycin) at current 60 mA with apparatus to electrotransfer (Biorad). .. Mouse polyclonal primary antibodies against HSP70 (Agrisera, AS09 592) at a 1:2,000 dilution (incubation for 75 min at room temp.) with goat anti-mouse horseradish peroxidase-conjugated secondary antibodies (Agrisera, AS09 602) at dilutions of 1:25,000 (incubation for 75 min at room temp.) were used.

    Article Title: Mycobacterium smegmatis l -Alanine Dehydrogenase (Ald) Is Required for Proficient Utilization of Alanine as a Sole Nitrogen Source and Sustained Anaerobic Growth
    Article Snippet: .. Proteins from a parallel preparation were electroblotted for 2 h at 45 V onto polyvinylidene difluoride membranes in electrotransfer buffer (10% methanol, Tris-glycine; pH 8.3) by using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad). ..

    Article Title: Mycobacterium smegmatis l -Alanine Dehydrogenase (Ald) Is Required for Proficient Utilization of Alanine as a Sole Nitrogen Source and Sustained Anaerobic Growth
    Article Snippet: Proteins from a parallel preparation were electroblotted for 2 h at 45 V onto polyvinylidene difluoride membranes in electrotransfer buffer (10% methanol, Tris-glycine; pH 8.3) by using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad). .. Proteins from a parallel preparation were electroblotted for 2 h at 45 V onto polyvinylidene difluoride membranes in electrotransfer buffer (10% methanol, Tris-glycine; pH 8.3) by using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad). .. Protein sequencing was performed with an ABIProcise 494 sequencer (Applied Biosystems, Foster City, Calif.) by using manufacturer-recommended protocols.

    Article Title: poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance
    Article Snippet: “Pull-down” and “sup” fractions were loaded into 4–15% Mini-PROTEAN TGX Precast protein gels (Biorad, 4561086) and run in Tris-glycine running buffer (25mM Tris, 192mM glycine, 0.1% SDS). .. Proteins were then transferred to nitrocellulose membrane (BioRad) at 100V for 1 hour in electrotransfer buffer (50mM Tris, 40mM glycine, 9% methanol, 0.2% SDS). .. Blotted membranes were blocked with 5% milk in PBST (phosphate-buffered saline, 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C.

    Article Title: Purification and characterization of an extracellular exochitinase, beta-N-acetylhexosaminidase, from the fungal mycoparasite Stachybotrys elegans.
    Article Snippet: The mycoparasite Stachybotrys elegans produces two exoand one endo-acting chitinases when grown on chitin.. We purified to homogeneity one of the exo-acting chitinases, β-N-acetylhexosaminidase and partially characterized its physical and biochemical properties.. The native enzyme has a molecular mass of 120 kDa when determined by gel filtration and 68 kDa by sodium dodecyl sulfate – polyacrylamide gel electrophoresis indicating that the native protein probably occurs as a dimer in solution.

    Article Title: Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco.
    Article Snippet: To elucidate the function of antifreeze protein from Microdera puntipennis dzhungarica for freezing stress tolerance in plant, the construct of MpAFP149 gene with the signal peptide sequence responsible for secreting the native MpAFP149 into the apoplast space under control of a cauliflower mosaic virus 35S promoter was introduced into tobacco by Agrobacterium tumefaciens-mediated transformation.. The observation of immunogold localization by TEM (transmission electron microscope) showed that the heterologous MpAFP149 protein was mainly distributed on the cell wall in apoplast of the transgenic tobacco plant.. T1 generation transgenic tobacco plants displayed a more frost resistant phenotype and kept the lower ion leakage ratio and MDA (malondialdehyde) content in the leaves compared with wild-type ones at -1 C for 3 days.

    Article Title: poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance
    Article Snippet: “Pull-down” and “sup” fractions were loaded into 4–15% Mini-PROTEAN TGX Precast protein gels (Biorad, 4561086) and run in Tris-glycine running buffer (25mM Tris, 192mM glycine, 0.1% SDS). .. Proteins were then transferred to nitrocellulose membrane (BioRad) at 100V for 1 hour in electrotransfer buffer (50mM Tris, 40mM glycine, 9% methanol, 0.2% SDS). .. Blotted membranes were blocked with 5% milk in PBST (phosphate-buffered saline, 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C.

    Article Title: Comparative proteomic analysis of virulent and rifampicin-attenuated Flavobacterium psychrophilum.
    Article Snippet: Flavobacterium psychrophilum is the aetiologic agent of bacterial coldwater disease and rainbow trout fry syndrome.. In this study, we compared a wild-type strain (CSF 259-93) with a rifampicin-resistant strain and virulence-attenuated strain of F. psychrophilum (CSF 259-93B.17).. The attenuated strain harboured a mutation in the rpoB gene consistent with resistance to rifampicin.

    Membrane:

    Article Title: poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance
    Article Snippet: “Pull-down” and “sup” fractions were loaded into 4–15% Mini-PROTEAN TGX Precast protein gels (Biorad, 4561086) and run in Tris-glycine running buffer (25mM Tris, 192mM glycine, 0.1% SDS). .. Proteins were then transferred to nitrocellulose membrane (BioRad) at 100V for 1 hour in electrotransfer buffer (50mM Tris, 40mM glycine, 9% methanol, 0.2% SDS). .. Blotted membranes were blocked with 5% milk in PBST (phosphate-buffered saline, 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C.

    Article Title: Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco.
    Article Snippet: To elucidate the function of antifreeze protein from Microdera puntipennis dzhungarica for freezing stress tolerance in plant, the construct of MpAFP149 gene with the signal peptide sequence responsible for secreting the native MpAFP149 into the apoplast space under control of a cauliflower mosaic virus 35S promoter was introduced into tobacco by Agrobacterium tumefaciens-mediated transformation.. The observation of immunogold localization by TEM (transmission electron microscope) showed that the heterologous MpAFP149 protein was mainly distributed on the cell wall in apoplast of the transgenic tobacco plant.. T1 generation transgenic tobacco plants displayed a more frost resistant phenotype and kept the lower ion leakage ratio and MDA (malondialdehyde) content in the leaves compared with wild-type ones at -1 C for 3 days.

    Article Title: poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance
    Article Snippet: “Pull-down” and “sup” fractions were loaded into 4–15% Mini-PROTEAN TGX Precast protein gels (Biorad, 4561086) and run in Tris-glycine running buffer (25mM Tris, 192mM glycine, 0.1% SDS). .. Proteins were then transferred to nitrocellulose membrane (BioRad) at 100V for 1 hour in electrotransfer buffer (50mM Tris, 40mM glycine, 9% methanol, 0.2% SDS). .. Blotted membranes were blocked with 5% milk in PBST (phosphate-buffered saline, 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C.



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